Journal: Journal of Orthopaedic Translation
Article Title: Construction of cartilaginous organoids based on cartilage extracellular matrix microcarriers to promote articular cartilage regeneration through immune regulation
doi: 10.1016/j.jot.2025.05.005
Figure Lengend Snippet: Effect of CEMMS on the polarization of rat macrophages. A) Quantitative analysis of M1 macrophage-related phenotypes using real-time PCR (n = 3). B) Quantitative analysis of M2 macrophage-related phenotypes using real-time PCR (n = 3). C) Immunofluorescence staining for markers of macrophages(CD86; Green)/(CD206; Red). Scale bars: 100 μm. D) Quantitative analysis of immunofuorescence staining. E) Immunofluorescence staining for markers of macrophages (Arg-1; Green)/(IL-1β; Red). Scale bars: 100 μm. F) Quantitative analysis of immunofuorescence staining. ∗∗∗∗ p < 0.0001.
Article Snippet: The cells were incubated with primary antibodies against CD86 (NBP2-25208, Novus), CD206 (24595S, CST), IL-1β(ab254360, Abcam) and Arg-1 (66129-1-Ig, proteintech) overnight at 4 °C.
Techniques: Real-time Polymerase Chain Reaction, Immunofluorescence, Staining